Cinética e parâmetros de fermentação ruminal in vitro de silagens de parte aérea e raízes de mandioca / Kinetic parameters and in vitro rumen fermentation of cassava foliage and roots silages

Foram avaliados os efeitos do emurchecimento e da inclusão de raízes 0, 15, 30 e 45% da matéria natural sobre a cinética e os parâmetros da fermentação ruminal de silagens da parte aérea da mandioca. A produção acumulada de gases (PAG) às 96 horas de incubação foi influenciada significativamente (P<0,05) pela interação emurchecimento versus inclusão de raízes. Para a PAG, houve elevação linear de 1,88mL a cada unidade percentual de raiz adicionada às silagens não emurchecidas e efeito quadrático nas silagens emurchecidas, com produção máxima de 226mL de gases, com a inclusão de 30,5% de raízes. A degradabilidade verdadeira in vitro da MS (DVIMS) das silagens apresentou significância (P<0,05) para o emurchecimento e para a interação inclusão de raízes versus tempo de incubação. O emurchecimento reduziu, aproximadamente, 2% da degradabilidade da silagem, enquanto a inclusão de raízes propiciou elevação linear dessa variável, que atingiu o valor máximo às 24 horas de incubação.

The effects of wilting and roots inclusion (0, 15, 30 and 45% fresh matter) on rumen fermentation kinetics and parameters of cassava foliage silages were evaluated. Total gas production (TGP) after 96 hours of incubation was significantly influenced (P <0.05) by the interaction between wilting and roots inclusion. TGP was linearly increased by 1.88 mL for each percent unit of root added to the wilted silage, whereas, for wilted ones, a quadratic effect was observed, with a maximum gas cumulative output of 226 mL for 30.5% roots addition. The true DM degradability of silages in vitro (IVTDMD) showed significance (P<0.05) to wilting and interaction between root inclusion and incubation time. Wilting reduced silage degradability in approximately 2%, while root inclusion led to linear increases in this variable, which reached its peak at 24h of incubation.

Analysis of acylcarnitine profiles in umbilical cord blood and during the early neonatal period by electrospray ionization tandem mass spectrometry

Acylcarnitine profiling by electrospray ionization tandem mass spectrometry (ESI-MS/MS) is a potent tool for the diagnosis and screening of fatty acid oxidation and organic acid disorders. Few studies have analyzed free carnitine and acylcarnitines in dried blood spots (DBS) of umbilical cord blood (CB) and the postnatal changes in the concentrations of these analytes. We have investigated these metabolites in healthy exclusively breastfed neonates and examined possible effects of birth weight and gestational age. DBS of CB were collected from 162 adequate for gestational age neonates. Paired DBS of heel-prick blood were collected 4-8 days after birth from 106 of these neonates, the majority exclusively breastfed. Methanol extracts of DBS with deuterium-labeled internal standards were derivatized before analysis by ESI-MS/MS. Most of the analytes were measured using a full-scan method. The levels of the major long-chain acylcarnitines, palmitoylcarnitine, stearoylcarnitine, and oleoylcarnitine, increased by 27, 12, and 109%, respectively, in the first week of life. Free carnitine and acetylcarnitine had a modest increase: 8 and 11%, respectively. Propionylcarnitine presented a different behavior, decreasing 9% during the period. The correlations between birth weight or gestational age and the concentrations of the analytes in DBS were weak (r £ 0.20) or nonsignificant. Adaptation to breast milk as the sole source of nutrients can explain the increase of these metabolites along the early neonatal period. Acylcarnitine profiling in CB should have a role in the early detection of metabolic disorders in high-risk neonates.

Analysis of acylcarnitine profiles in umbilical cord blood and during the early neonatal period by electrospray ionization tandem mass spectrometry.

Acylcarnitine profiling by electrospray ionization tandem mass spectrometry (ESI-MS/MS) is a potent tool for the diagnosis and screening of fatty acid oxidation and organic acid disorders. Few studies have analyzed free carnitine and acylcarnitines in dried blood spots (DBS) of umbilical cord blood (CB) and the postnatal changes in the concentrations of these analytes. We have investigated these metabolites in healthy exclusively breastfed neonates and examined possible effects of birth weight and gestational age. DBS of CB were collected from 162 adequate for gestational age neonates. Paired DBS of heel-prick blood were collected 4-8 days after birth from 106 of these neonates, the majority exclusively breastfed. Methanol extracts of DBS with deuterium-labeled internal standards were derivatized before analysis by ESI-MS/MS. Most of the analytes were measured using a full-scan method. The levels of the major long-chain acylcarnitines, palmitoylcarnitine, stearoylcarnitine, and oleoylcarnitine, increased by 27, 12, and 109%, respectively, in the first week of life. Free carnitine and acetylcarnitine had a modest increase: 8 and 11%, respectively. Propionylcarnitine presented a different behavior, decreasing 9% during the period. The correlations between birth weight or gestational age and the concentrations of the analytes in DBS were weak (r ≤ 0.20) or nonsignificant. Adaptation to breast milk as the sole source of nutrients can explain the increase of these metabolites along the early neonatal period. Acylcarnitine profiling in CB should have a role in the early detection of metabolic disorders in high-risk neonates.

Fracionamento de carboidratos e proteínas de gramíneas tropicais cortadas em três idades / Carbohydrates and proteins fractions of tropical grasses cut at three ages

Avaliou-se o fracionamento dos carboidratos e das proteínas das gramíneas tropicais tifton 85 (Cynodon dactylon cv Tiffon 85), braquiária (Brachiaria brizantha cv Marandu) e tanzânia (Panicum maximum Jacq. cv Tanzânia), cortadas aos 28, 35 e 54 dias de idade. Determinaram-se os teores de matéria seca (MS), matéria orgânica (MO), proteína bruta (PB), extrato etéreo (EE), fibra em detergente neutro corrigida para cinzas e proteína (FDN CP), fibra em detergente ácido (FDA), lignina (LIG), nitrogênio insolúvel em detergente neutro (NIDN) e nitrogênio insolúvel em detergente ácido (NIDA). Para PB determinaram-se as frações A, B1+B2, B3 e C, e para os carboidratos as frações A+B1, B2 e C. Foi utilizado o delineamento inteiramente ao acaso, em arranjo em parcelas (gramíneas) subdivididas (idades de corte). Houve efeito significativo (P<0,05) da idade de corte sobre os teores de MS, FDN CP, NIDN e NIDA. Os teores de PB apresentaram declínio com o avanço da idade. O capim-marandu apresentou teores elevados para as frações A+B1 e B2 dos carboidratos (25,5 e 34,8 por cento dos carboidratos totais, respectivamente) e para as frações A e B1+B2 das proteínas (28,1 por cento aos 35 dias e 53,8 por cento da PB aos 28 dias, respectivamente).

The carbohydrates and proteins fractions of tropical grasses Tifton 85 (Cynodon dactylon cv Tifton 85), brachiaria (Brachiaria brizantha cv Marandu), and Tanzania (Panicum maximum Jacq. cv Tanzania) were evaluated at cut ages of 28, 35, and 54 days. Contents of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), fiber in neutral detergent corrected for ashes and protein (NDF CP), fiber in acid detergent (ADF), lignin (LIG), neutral detergent insoluble nitrogen (NDIN), and acid detergent insoluble nitrogen (ADIN) were determined. For CP, the A, B1+B2, B3, and C fractions were determined. For carbohydrates, the fractions A+B1, B2, and C were measured. A completely randomized split-plot design was used. Effect of cut age on DM, NDF CP, NDIN and ADIN was observed. The CP contents decreased with the progress of the age for the three grasses. The Marandu grass presented high percentage of the fractions A+B1 and B2 for the total carbohydrate (25.5 and 34.8 percent, respectively) and of the fractions A and B1+B2 for the proteins (28.1 percent CP at 35 days and 53.8 percent CP at 28 days, respectively).

Avaliação da Brachiaria brizantha cv. marandu em sistemas silvipastoris / Evaluation of Brachiaria brizantha cv. marandu in silvopastoral systems

Avaliou-se a degradabilidade in situ da matéria seca (MS) da Brachiaria brizantha cv. marandu colhida em dois sistemas silvipastoris compostos pelas arbóreas, ipê felpudo (Zeyheria tuberculosa) e aroeira (Myracrodruon urundeuva), localizados no bioma Cerrado, município de Lagoa Santa, MG. O período de coleta da forragem foi de dezembro de 2004 a abril de 2005. O delineamento utilizado foi em blocos ao acaso, segundo esquema de parcelas subdivididas, sendo as parcelas os tratamentos e as sub-parcelas os tempos de degradação. O sombreamento com as arbóreas não influenciou a degradabilidade in situ da matéria seca da forrageira.

In situ degradability of dry matter of Brachiaria brizantha cv. marandu harvested in two silvopastoral systems was evaluated. One system was composed by Zeyheria tuberculosa tree and the other by Myracrodruon urundeuva, both located at Brazilian Savannah, Lagoa Santa, MG. A completely ramdomized block experimental design in a splitplot arrangement was used. The treatments were allocated in the plots and the times in the splitplot. No effect of shade was observed for in situ dry matter degradability.